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celltrace tm violet cell proliferation kit (Thermo Fisher)

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Thermo Fisher celltrace tm violet cell proliferation kit

(A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). <t>CellTrace</t> Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.

Celltrace Tm Violet Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltrace+tm+violet+cell+proliferation+kit/celltrace+violet/pmc12281710-105-0-10
Average 90 stars, based on 1 article reviews

celltrace tm violet cell proliferation kit - by Bioz Stars, 2026-09

90/100 stars

Images

1) Product Images from "Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells"

Article Title: Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells

Journal: Cell reports

doi: 10.1016/j.celrep.2025.115832

Figure Legend Snippet: (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). CellTrace Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.

Techniques Used: Transplantation Assay, Microscopy, Labeling, Confocal Microscopy, Two Tailed Test

Staining:

Article Title: A novel anti-membrane CD30 single-chain variable fragment discovered from the human phage library: A potential targeted immunotherapy
Article Snippet: .. CD30+ cell lines, K562 cell lines, and CD30- cell line, SupB15, were stained with 1μM of CellTrace TM Violet cell proliferation kit (Life Technologies Corporation, ThermoFisher Scientific, USA) and co-cultured individually with engineered- and mock T cells at effector-to-target ratios (E: T) of 1:1 and 40:1. .. After 24 hours of culture, the cell pellet was stained with 7-amino-actinomycin D (7AAD) (eBioscience, USA) to detect the proportion of dead target cells and analyzed by Navios Flow Cytometry (Beckman Coulter, USA) and FlowJo software (version 10, TreeStar, USA).

Article Title: Microbiota-derived butyrate inhibits cDC development via HDAC inhibition, diminishing their ability to prime T cells
Article Snippet: Naïve OVA-specific CD4 + T cells were purified from the spleen and lymph nodes of OTII TCR transgenic mice using the MojoSort TM Mouse CD4 Naïve T cell isolation Kit according to the manufacturer’s instructions (Biolegend). .. Cells were then stained with the CellTrace TM Violet Cell proliferation kit (ThermoFisher) according to the manufacturer’s instructions. .. FACS-isolated colonic cDC populations or in vitro generated DC were pulsed with OVA protein (#SLBS4311, Sigma) at 2mg/mL for 2 h at 37°C and co-cultured with T cells at 1:10 ratio for 3 days in complete RPMI in a total volume of 300μl in round-bottomed 96-well plates (Costar, #3799).

Flow Cytometry:

Article Title: Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells
Article Snippet: CellTracker TM CM-Dil , Thermo Fisher , Cat # C7000. .. CellTrace TM Violet Cell Proliferation Kit, for flow cytometry , Thermo Fisher , Cat # C34557. .. CellTrace TM Far Red Proliferation Kit, for flow cytometry , Thermo Fisher , Cat # C34564.

Article Title: Transcription factor RORα enforces stability of the Th17 cell effector program by binding to a Rorc cis -regulatory element
Article Snippet: EdU Flow Cytometry 647–50 Kit + EdU , Baseclick , Cat. BCK647-IV-FC -M. .. CellTrace TM Violet Cell Proliferation Kit, for flow cytometry , ThermoFisher , Cat. C34557. .. EasySep TM Mouse CD90.1 Positive Selection Kit , STEMCELL , Cat. 18958.

Control:

Article Title: A Novel FACS-Based Workflow for Simultaneous Assessment of RedOx Status, Cellular Phenotype, and Mitochondrial Genome Stability
Article Snippet: Anti-CD105-PE (eBiosciences, San Diego, CA, USA; clone SN6) was used to determine MSC phenotype. .. For PBMC experiments, the 4 treatment groups were first barcoded according to EtBr treatment using CellTrace TM Violet Cell Proliferation Kit (ThermoFisher Scientific, Waltham, MA, USA) as follows: Control (0.04 μM), 400 ng/mL EtBr (0.16 μM), 1200 ng/mL EtBr (0.63 μM) and IL-15 (2.5 μM). ..

Image Search Results

Journal: Cell reports

Article Title: Transplants foster B cell alloimmunity by relaying extracellular vesicles to follicular dendritic cells

doi: 10.1016/j.celrep.2025.115832

Figure Lengend Snippet: (A) Donor H2 Ag in splenic FDCs (inset, arrows) after transplantation of B6 (H2 b ) hearts in BALB/c mice. Original magnification ×200. Scale bars, 20 μm. (B) Quantification with ImageJ of donor H2 Ag spots per FDC network on spleens of BALB/c mice transplanted with B6 hearts. (C) Donor H2 Ag spots per FDC network on BALB/c LNs draining B6 skin allografts assessed using ImageJ. (D) Deep SIM of donor H2 Ag spots in FDCs of a BALB/c LN draining a B6 skin graft. Scale bar, 2 μm. ROI, region of interest. (E and F) 2P-microscopy of FDCs in the spleen of a BALB/c mouse transplanted with a B6 heart releasing RFP-sEVs (E) and a BALB/c LN draining a B6 skin graft releasing RFP-sEVs (F). Arrows: graft-RFP-sEVs on the surface (red) or inside (yellow) of FDCs (green). CellTrace Violet-B cells indicate B cell follicles. FDCs were labeled with AF488-CD21/CD35 Ab. Original magnification ×25. Scale bars, 20 μm. (G) Colocalization by confocal microscopy of donor (B6, IA b + H2K b ) allo-Ags in FDCs of LNs draining CMV Cre/+ LSL -RFP-CD63 B6 skin grafted in BALB/c mice. Scale bar, 10 μm. Mander’s colocalization coefficient of graft-sEVs and donor IA b + H2K b Ab analyzed using Imaris. Each triangle represents one FDC network. In (E) and (F), RFP spots per FDC network z stacks were quantified within 90-μm-thick z stacks using Imaris. Experiments were done on 4 spleens per POD (A and B), 6 graft-dLNs per POD (C), 3 graft-dLNs (D), 4 spleens (E), 4 graft-dLNs (F), and 12 graft-dLNs (G). In (B), (C), (E), and (F), comparisons were made by multiple unpaired two-tailed Student’s test. Error bars, means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; NS, not significant.

Article Snippet: CellTrace TM Violet Cell Proliferation Kit, for flow cytometry , Thermo Fisher , Cat # C34557.

Techniques: Transplantation Assay, Microscopy, Labeling, Confocal Microscopy, Two Tailed Test

(A) C57BL/6 mice (n=4 for each day) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed in kinetic. The percentage of Sca1+, LAG3+ and CD69+ splenic NK cells was analyzed by flow cytometry. (B) The percentage of LAG3+ among Sca1+ and Sca1-NK cells on Day2 was analyzed by flow cytometry (n=5). (C-G) C57BL/6 mice (n=2 for each experiment) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed on Day2. Splenic NK cells were stained for the phospho-epitope pS6 Ser235/236 (C) and for pAkt S473 (D). Overlays of representative histograms are shown (left panels). The MFI values are indicated on the graph and represented on the right panel for each individual, n=6, 3 experiments in total. (E) NK cells were stained with Mitotracker Green and CellRox. The MFI values for each mouse (n=7, 3 experiments in total) for the analyzed marker are represented. (F) The MFI of CD98 (left) or CD71 (right) was determined by flow cytometry on LAG3+ and LAG3-NK cells (n=3). Due to the small sample size, we did not calculate p-values. (G) SSC-A and FSC-A parameters on NK cells were measured by flow cytometry in LAG3- and LAG3+ NK cells (n=5). (H) Splenic NK cells from C57BL/6 mice (n=6, 3 experiments) were isolated, stained with Cell Trace Violet (CTV) and cultured with 100ng/mL of IL-15 for 3 days. The MFI of CTV was analyzed by flow cytometry and the overlays of representative histograms are shown (left panel). The percentage of divided NK cells for each individual was calculated using the proliferation modeling tool on the FlowJo software. All the data were analyzed using a paired non-parametric Wilcoxon test. p-values are indicated on each graph.

Journal: bioRxiv

Article Title: LAG3 marks activated but hyporesponsive NK cells

doi: 10.1101/2024.12.18.629184

Figure Lengend Snippet: (A) C57BL/6 mice (n=4 for each day) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed in kinetic. The percentage of Sca1+, LAG3+ and CD69+ splenic NK cells was analyzed by flow cytometry. (B) The percentage of LAG3+ among Sca1+ and Sca1-NK cells on Day2 was analyzed by flow cytometry (n=5). (C-G) C57BL/6 mice (n=2 for each experiment) were injected intraperitoneally with 200ug of Poly (I:C) and sacrificed on Day2. Splenic NK cells were stained for the phospho-epitope pS6 Ser235/236 (C) and for pAkt S473 (D). Overlays of representative histograms are shown (left panels). The MFI values are indicated on the graph and represented on the right panel for each individual, n=6, 3 experiments in total. (E) NK cells were stained with Mitotracker Green and CellRox. The MFI values for each mouse (n=7, 3 experiments in total) for the analyzed marker are represented. (F) The MFI of CD98 (left) or CD71 (right) was determined by flow cytometry on LAG3+ and LAG3-NK cells (n=3). Due to the small sample size, we did not calculate p-values. (G) SSC-A and FSC-A parameters on NK cells were measured by flow cytometry in LAG3- and LAG3+ NK cells (n=5). (H) Splenic NK cells from C57BL/6 mice (n=6, 3 experiments) were isolated, stained with Cell Trace Violet (CTV) and cultured with 100ng/mL of IL-15 for 3 days. The MFI of CTV was analyzed by flow cytometry and the overlays of representative histograms are shown (left panel). The percentage of divided NK cells for each individual was calculated using the proliferation modeling tool on the FlowJo software. All the data were analyzed using a paired non-parametric Wilcoxon test. p-values are indicated on each graph.

Article Snippet: Isolated NK cells were stained with 0.6µM Invitrogen CellTrace TM Violet (CTV) Cell Proliferation Kit according to manufacturer’s instructions.

Techniques: Injection, Flow Cytometry, Staining, Marker, Isolation, Cell Culture, Software